Journal: World Journal of Surgical Oncology

Article Title: A study of the correlation between M2 macrophages and lymph node metastasis of colorectal carcinoma

doi: 10.1186/s12957-021-02195-5

Figure Lengend Snippet: Expression of M2 macrophages in lymph nodes. Detection of CD163 in lymph nodes of colorectal carcinoma patients by immunohistochemistry. The membrane and cytoplasm of M2 macrophages are stained brown. Microscopic analysis of a typical example of CD163 expression in non-metastatic lymph nodes ( a , b , c magnification × 100, × 200, × 400, respectively). d , e Location of CD163 in metastatic lymph node tissue at a magnification of × 200 and × 400 respectively. M2 macrophages are seen mainly infiltrating into the tumor stroma

Article Snippet: The tissue sections were incubated with an endogenous peroxidase blocker at room temperature for 10 min, blocked with 3% goat serum (cat. No. KIT-9710; Maixin-Bio, Fuzhou, China) for 30 min, rinsed with phosphate-buffered saline (PBS), incubated with ready-to-use mouse anti-human CD163 monoclonal antibody (cat. No. MAB-0206; Maixin-Bio) at room temperature for 60 min, followed by sequential addition of biotin-labeled IgG polymer and streptavidin peroxidase (cat. No. KIT-9710; Maixin-Bio).

Techniques: Expressing, Immunohistochemistry, Staining